GlpF 3D test data

This directory contains 9 TIFF images as test images for the 2dx processing and merging.

Size     Date         File Name
36018144 Aug 30 12:39 glpf0000655301.tif
36018048 Aug 30 12:40 glpf0000655501.tif
16794268 Aug 30 12:40 glpf0000655701.tif
31380264 Aug 30 12:40 glpf3000665301.tif
31739840 Aug 30 12:40 glpf3000666701.tif
31380088 Aug 30 12:41 glpf3000669001.tif
73635340 Aug 30 12:41 glpf4500800501.tif
56088780 Aug 30 12:41 glpf4500801301.tif
43578232 Aug 30 12:41 glpf4500802501.tif

These images are cryo-EM images of the Glycerol Facilitator Protein F from E.coli (GlpF). 
2D crystals were grown by Thomas Braun.
Images were recorded on film by Thomas Braun and Henning Stahlberg on the JEOL JEM-3000SFF 
in the MPI Frankfurt (Kuehlbrandt), using SpotScanning illumination. 
The images were digitized with a Zeiss scanner. 

The first three images are from non-tilted samples.
Magnification is 70.000x, digitization was done from these films at 7 micrometer/pixel. Cs=1.6mm, HV=300kV.

The next three images are from 30-deg tilted samples.
Magnification is 50.000x, digitization was done from these films at 7 micrometer/pixel. Cs=1.6mm, HV=300kV.

The last three images are from 45-deg tilted samples.
Magnification is 70.000x, digitization was done from these films at 7 micrometer/pixel. Cs=1.6mm, HV=300kV.

The tilt axis for the tilted images should be roughly horizontal.