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Electron Crystallography Workshop 2010

    Aims

    The workshop on membrane protein 2D crystallization and electron crystallography is aimed at everybody (PhD students, PostDocs, group leaders, etc.), who is interested in the structure determination of membrane proteins (or other 2D protein crystal arrays) by electron crystallography.

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    Topics

    This workshop has two major aims:

    1. Advancing the technology: To bring together many of the leading groups in electron crystallography to forge innovative collaborations for new technology development.

    2. Training: To provide a unique forum to train skilled biochemists and electron microscopists (PhD, Post docs and beyond), in membrane protein 2D crystallization, electron crystallographic data collection and data processing.

      • Membrane protein solubilisation and crystallization
      • Sample preparation for electron microscopy
      • Cryo-EM data collection (imaging and electron diffraction)
      • Data processing with the MRC, IPLT and 2dx software packages
      • Data evaluation and model building

       

    Workshop format

    The workshop will feature lectures in the morning, practicals in the afternoon, and science talks in the evening. Membrane protein crystallization trials will introduce lipid monolayer crystallization methods, or 2D crystallization with dialysis devices, or with a cyclodextrin robot. We will demonstrate automated 2D crystal screening, and present cryo-EM imaging and electron diffraction on the CM200FEG and Titan Krios. We will lecture image processing using MRC, IPLT and 2DX software systems. Computer practicals will use machines in a local computer room. However, students are also invited to bring their own laptop computer (Linux or OSX), where we can try to install the image processing software packages and run the processing on your own computers during the workshop.

    Speakers (confirmed)

    • Anchi Cheng, Scripps, La Jolla
    • Andreas Engel, C-CINA, Basel
    • Andreas Schenk, Harvard Med. School, Boston
    • Carsten Sachse, EMBL, Heidelberg
    • Catherine Venien-Bryan, Univ. Oxford, UK
    • Clemens Schulze-Briese, PSI, Villigen
    • Daniel Levy, Inst. Curie, Paris
    • Dimitrios Fotiadis, Bern, Switzerland
    • Hans Hebert, Karolinska, Stockholm
    • Henning Stahlberg, C-CINA, Basel
    • Iban Ubarretxena-Belandia, Mount Sinai, NY
    • Janet Vonck, MPI Frankfurt, Germany
    • Jürgen Plitzko, MPI Martinsried, Germany
    • Marcel Arheit, C-CINA, Basel
    • Michael Landsberg, Univ. Queensland, Brisbane
    • Nicolas Coudray, Univ. Mulhouse, France
    • Nobuhiko Gyobu, AIST, Tokyo
    • Per Bullough, Univ. Sheffield, UK
    • Rafael Abela, SwissFEL, PSI, Villigen
    • Richard Henderson, MRC, Cambridge, UK
    • Richard Hite, Harvard Med. School, Boston
    • Tom Walz, Harvard Med. School, Boston
    • Valerio Mariani, Biozentrum, Basel
    • Werner Kühlbrandt, MPI Frankfurt
    • Xiangyan Zeng, FVSU, Georgia, USA

    Dates

    Workshop Lectures: August 2-6, 2010 (Monday-Friday) Arrival: Sunday, August 1, 2010. Departure: Saturday, August 7, 2010

    Program

    The workshop will be divided into three blocks: Lectures (morning), hands-on practicals (afternoon), and research talks (evening). To complete program is available here.

    C-CINA (Centre for Cellular Imaging and Nano Analytics)
    Biozentrum, University Basel
    WRO-1058
    Mattenstrasse 26
    CH-4058 Basel
    Switzerland

    Geographically, the lab is located in the Department of Biosystems Science and Engineering (D-BSSE), which is a branch of the Swiss Federal Institute of Technology (ETHZ).
    A description how to find the C-CINA laboratory can be found here.

    For a virtual map click here.

    Travel

    Travel to and from the workshop at C-CINA has to be organized by the participants. The closest airport is Euroairpor Basel-Mulhouse-Freiburg from which you can take a bus and arrive within 10 minutes in the city. If you arrive at Zürich Airport it is an hour train ride from Basel. We would recommend you take the train then. You find the schedule and ticket prices on the SBB website.

    Lodging

    Lodging is not included in the registration fee. We have made some reservations for "Biozentrum Uni Basel" at the following hostels:

    Rooms may also be shared.

    Registration

    Registration has been terminated.

    The workshop is currently limited to 30 participants. The fee for academic participants is CHF 300.-, which covers registration, breakfast and lunch during the workshop. Lodging is not included in the registration fee.

     

    Festival Before Workshop

    If you like to spend some days in Switzerland before the workshop starts, you could

    • enjoy the wonderful nature of Switzerland (no car needed, public transport is excellent), or the historic city of Basel,
    • look at the BlueBalls festival in Luzern, one hour by train from Basel, see here.
    • or look at the Stimmen festival in Loerrach, Germany, just accross the border a few minutes by public transport from Basel, see here.
    • or on July 31, the night before the workshop, join the celebration for the Swiss National Festival with a big firework at 22:40, see here.
    • or visit the Fondation Beyeler and see the Basquiat Exhibition, see here.
    That should get you over the jetlag....

     

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